Biotium
XTT Cell Viability Assay Kit
30007
Info:Supplier Page
I study gene signaling pathways and routinely measure how genetic or chemical perturbations affect cell viability. This XTT assay provides a straightforward colorimetric readout that is easy to use in a 96-well format.
Cell viability and proliferation assays
Cultured mammalian cells
Perform an optimization step by reading the absorbance at various time points
The assay produces a water-soluble orange product, so there is no additional solubilization step as with MTT reagent. I measure the signal by absorbance at 465 nm and background absorbance at 630 nm using a standard plate reader.
Simple workflow, no solubilization step, easy optimization
Less sensitive than some fluorescent or luminescent viability assays
A convenient and easy-to-use viability assay for routine cell-based experiments