Quick and Simple DNA Extraction for Genotyping

Stanford University
Department of Pathology
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Questions/Comments

Company:

LGC Biosearch Technologies

Product Name:

QuickExtract DNA Extraction Solution

Catalog Number:

QE0905T

I study gene signaling pathways and routinely work with genetically modified cells. QuickExtract makes DNA preparation fast and convenient for screening edited clones and checking genotypes.

Experimental Design and Results Summary

Application

DNA extraction

Starting Material

Cell pellet

Tips

Aliquot and freeze for long term storage

Results Summary

I used 5000-10000 cells with 10-20 ul buffer for quick DNA extraction. Vortex to resuspend the pellet in extraction buffer before incubation. The single-tube protocol takes few minutes of incubation and requires no spin columns or centrifugation.

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Summary

The Good

requires very less sample, compatible with downstream PCR

The Bad

None

The Bottom Line

Extremely fast, simple, and convenient genomic DNA extraction method for high-throughput genotyping.

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