Abcam
Anti-Lamin A antibody - Nuclear Envelope Marker
ab26300
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I study gene signaling pathways and use this antibody as a nuclear envelope marker in cell fractionation experiments and western blots. This antibody provides clean, specific band for Lamin A.
Western Blot
Cell lysate and cell fractionation samples
1:1000, overnight, 4C
5% Milk in TBST
1:10000, 1 hr, RT
NA
ECL
I obtained a clear Lamin A band in whole cell and nuclear fractions at the expected size. Any non-specific band was not seen in cytoplasmic fraction.
KO validated, reliable signal
None
A useful Lamin A antibody that works great as a nuclear marker.