Lamin A Antibody That Works Great as a Nuclear Marker

Department of Pathology
Stanford University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-Lamin A antibody - Nuclear Envelope Marker

Catalog Number:

ab26300

I study gene signaling pathways and use this antibody as a nuclear envelope marker in cell fractionation experiments and western blots. This antibody provides clean, specific band for Lamin A.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Cell lysate and cell fractionation samples

Primary Incubation

1:1000, overnight, 4C

Blocking Agent

5% Milk in TBST

Secondary Incubation

1:10000, 1 hr, RT

Tertiary Incubation

NA

Detection

ECL

Results Summary

I obtained a clear Lamin A band in whole cell and nuclear fractions at the expected size. Any non-specific band was not seen in cytoplasmic fraction.

Image Gallery

Summary

The Good

KO validated, reliable signal

The Bad

None

The Bottom Line

A useful Lamin A antibody that works great as a nuclear marker.

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