Best PCR Master Mix for Consistent Results

Stanford University
Department of Pathology
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Questions/Comments

Company:

Invitrogen

Product Name:

Platinum™ SuperFi II PCR Master Mix

Catalog Number:

12369010

I routinely genotype cell culture lines and amplify from genomic DNA. This high-fidelity PCR master mix is useful for difficult-to-amplify targets when sequence accuracy is important for validating genetic constructs.

Experimental Design and Results Summary

Application

PCR

Starting Material

genomic DNA

Tips

The master mix uses a universal primer annealing temperature of 60°C

Results Summary

The master mix works consistently and reliably to amplify difficult genomic DNA targets. The green dye version can be loaded directly onto agarose gels.

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Summary

The Good

high-fidelity, 15-30 seconds/kb, single annealing temperature

The Bad

Expensive for doing large-scale genotyping

The Bottom Line

A great choice for quick, reliable and accurate amplification.

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