Good HA-Tag Antibody for IF

Department of Pathology
Stanford University
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Purified anti-HA.11 Epitope Tag Antibody

Catalog Number:

902301

I study gene signaling pathways and use immunofluorescence to image cultured cells. This BioLegend antibody works well for staining HA-tagged proteins and provides clear staining.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Transfected mammalian cells

Primary Incubation

1:500, 1 hour, Room temperature

Blocking Agent

Donkey serum and BSA

Secondary Incubation

1:500, 1 hour, Room temperature

Tertiary Incubation

DAPI in mounting media

Detection

Confocal microscopy

Results Summary

I obtained clear staining and bright fluorescence signal for the cells expressing HA-tagged protein, making the antibody useful for assessing cellular protein localization.

Image Gallery

Summary

The Good

Specific and clean signal

The Bad

None

The Bottom Line

A good antibody for HA-tag detection.

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