BD Biosciences
Purified Mouse Anti-β-Catenin antibody
610154
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I study gene signaling pathways and use β-catenin to investigate cell adhesion and Wnt signaling. This monoclonal antibody is useful for detecting β-catenin in cultured cells.
Immunofluorescence
PFA-fixed human cell lines
1:200, 1 hour, Room temperature
Donkey serum and BSA
1:500, 1 hour, Room temperature
DAPI in mounting media
Confocal microscopy
The antibody gives very good signal for staining in microscopy. Beta-catenin is mostly membrane bound in HT29 cancer cell line.
can be used for western blots and IP
None
Useful antibody for examining β-catenin expression and localization.