Vanessa Kelley

Graduate Student

Yale University

Great Reagent for Cell Cycle Analysis
July 25, 2022
We wanted to examine changes in cell cycle when we knocked out a gene of interest in the presence and absence of radiation. We ... read more
Great Mounting Medium for Clear Fluorescence Signal
July 25, 2022
We wanted to examine Rad51 foci formation in the Cal27 Head and Neck Cancer cell line after 4Gy of radiation treatment. read more
Great Kit for Protein Quantification
July 25, 2022
We use this kit routinely to quantify the amount of protein in cell lysates to be used in western blots. The kit is easy to use ... read more
Good BIM Antibody for Upper Isoform Quantification
June 22, 2022
We knocked out an upstream JAK protein (JAK KO) and wanted to examine if there were changes in BIM expression in JAK KO cells. We ... read more
Excellent Antibody for Phospho-Y1068 EGFR Signal
June 22, 2022
We generated a knockout of a member of the JAK family of proteins and wanted to examine whether there were changes in EGFR ... read more
Good Phospho-STAT3 Antibody for Protein Quantification
June 15, 2022
We knocked out an upstream JAK protein in the Cal27 cell line and wanted to examine downstream STAT3 signaling. We blotted JAK KO ... read more
Good Ku80 Antibody for Total Protein Quantification
May 31, 2022
We knocked out a member of the JAK protein family in two head and neck cancer cell lines, and we wanted to determine if there were... read more
Good Antibody for cGAS Localization
May 24, 2022
We wanted to determine whether cGAS was localized to micronuclei in head and neck cancer, as this was previously described in ... read more
Excellent Antibody for IRF9 Protein
May 12, 2022
We generated a genetic knockout of an upstream JAK protein and wanted to determine whether the JAK knockout cells had decreased ... read more
Excellent Total STAT3 Antibody
May 11, 2022
This is an excellent antibody for detection of the STAT3 protein. We used CRISPR-Cas9 to knockout a JAK protein that is upstream ... read more
Good JAK1 Antibody for Total Protein Quantification
April 29, 2022
I generated a JAK1 knockout cell line and wanted to confirm that the JAK1 protein was completely depleted from these cells using a... read more

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Detection of LAP+ Treg Cells by Flow Cytometry

Mitesh Dwivedi

The aim of the experiment was to detect LAP+ CD4+CD25+ regulatory T cells (Tregs) isolated from whole blood of humans. read more

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