Cell Signaling
IRF9 Antibody
76684S
D2T8M
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We generated a genetic knockout of an upstream JAK protein and wanted to determine whether the JAK knockout cells had decreased IRF9 expression. We tested the levels of IRF9 in the presence and absence of radiation at several time points after treatment with 4Gy of radiation. We saw that the JAK knockout cells had a decrease in IRF9 expression regardless of the presence or absence of radiation treatment.
Western Blot
Cal27
1:1000 dilution of primary antibody in 1% BSA incubated overnight at 4 degrees celsius on a slow rocker.
5% milk
1:1000 goat-anti-rabbit HRP conjugated antibody suspended in 1% milk for one hour at room temperature on a slow rocker.
N/A
Film
We found that the JAK knockout cells had a global decrease in IRF9 expression regardless of radiation treatment. The IRF9 bands were clear with a crisp signal in the expected size range.
Excellent antibody signal with robust bands in the expected size range.
The antibody does not re-use well, even with the addition of sodium azide to the primary solution.
Excellent antibody for IRF9 with good signal.