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Date Published
Detection Of PCNA In Primary Tumor Cells By Immunoblotting
(1)
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Novus Biologicals, a Bio-Techne Brand
February 02, 2017
The aim of this study was to quantify PCNA protein expression in primary mammary tumor cells isolated from transgenic mouse mammary glands. ...
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IL-6 (D5W4V) XP® Rabbit MAb (Mouse Specific)
(1)
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Cell Signaling Technology
February 01, 2017
I am studying molecular anomalies in signaling pathways leading to cancers and development of anti cancer immune therapies.
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GAPDH Antibody
(1)
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February 01, 2017
The general aim was to quantify levels of various endogenous proteins after treatment with siRNA. The goal was to use GAPDH as a loading ...
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Cleaved Caspase 3 Antibody
(1)
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Cell Signaling Technology
February 01, 2017
We have used this antibody successfully on Western blots to look at active caspase 3, this is the one we find to work best. When we were ...
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Good ABC-AP Staining Kit
(1)
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February 01, 2017
I used this kit for staining after using an anti-cytokeratin 8-18 primary guinea pig Ab (sensitive against mouse) against preneoplastic ...
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RT-PCR Using Invitrogen's SuperScript III (SSIII) Synthesis System
(1)
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Thermo Fisher Scientific
February 01, 2017
We employed Invitrogen's SSIII RT-PCR kit to generate cDNA from mRNA that was previously isolated from whole blood using traditional phenol-...
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Easy, Fast, High Yield Midiprep
(1)
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Zymo Research
February 01, 2017
I’m studying regulatory sequences in embryonic stem cells. This product was used to clone reporter plasmids to test potential regulatory ...
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Detect Sox9 In Mouse Embryonic Stem Cells
(1)
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R&D Systems, a Bio-Techne Brand
February 01, 2017
The aim of this experiment was to detect the expression of Sox9 in fixed mouse embryonic stem cells. The goal was to observe Sox9 positive-...
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Lipofectamine 3000 Reagent from Thermo Fisher Scientific
(1)
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Thermo Fisher Scientific
January 31, 2017
In our lab, we use Lipofectmamine 3000 reagent as our primary transfection reagent for a wide range of experiments. I, personally, have used...
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Isotype Control For The Detection Of Non-Specific Binding Of PE Rat Anti-Mouse CD117
(1)
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January 31, 2017
The aim of this experiment was to use the PE Rat IgG2b as isotype control to detect the non-specific binding of PE Rat anti-mouse CD 117 ...
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Cell Fractionation Kit Review
(1)
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Abcam
January 31, 2017
We bought this kit thinking it would be an easy way to run a subcellular fractionation, to repeat data from a paper that we saw. We followed...
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Quantification Of Human PF-4 In Serum, Plasma Using An ELISA Kit
(1)
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Thermo Fisher Scientific
January 31, 2017
The Human PF-4 ELISA Kit can be used to detect and quantify PF-4 levels in biological fluids. PF-4 is released from the alpha granules of ...
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Mouse IL-4 Antibody
(1)
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R&D Systems, a Bio-Techne Brand
January 31, 2017
I am studying molecular anomalies in signaling pathways leading to cancers and development of anti cancer immune therapies.
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Immunofluorescence Of IP3R For Proximity Ligation Assay
(1)
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Abcam
January 31, 2017
The mitochondrial associated membrane (MAM) is a well established point of communication between the ER and mitochondria. IP3R is typically ...
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Cheap Easy Homemade Competent Cells
(1)
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Zymo Research
January 31, 2017
I’ve tried some other homemade competent cell protocols, but have got higher efficiency cells using the Zymo kit. Additionally, you can skip...
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Isotype Control For The Detection Of Non-Specific Binding Of APC Rat Anti-Mouse CD184
(1)
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January 30, 2017
The APC Rat IgG2b, was used at the isotype control for the detection of APC CD184 using flow cytometry. The overall objective of this ...
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Easy Assay To Measure LDH Levels In Cell Supernatant
(1)
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Promega
January 30, 2017
This assay is super easy and can easily be coupled to other assays, as you only take the supernatant from the cells to look at released LDH....
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Easy Dual Luciferase Assays
(1)
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Molecular Devices
January 30, 2017
I use this for dual luciferase assays. It is super easy. Just need to hook up your two reaction buffers in separate tubes and put in the ...
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VDAC For Proximity Ligation Assay
(1)
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Abcam
January 30, 2017
The mitochondrial associated membrane (MAM) is now an established point of communication between the ER and mitochondria. VDAC is typically ...
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FITC-Conjugated Anti-Rat CD3 (Clone G4.18) Is Not Cross-Reactive With Human CD3
(1)
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BD Biosciences
January 30, 2017
Our lab routinely tests antibodies for cross-reactivity to other species for use in immunological assays. This antibody which recognizes rat...
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Isotype Control for K9 IP Experiment
(1)
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BioLegend
January 30, 2017
The aim of this experiment is to detect presence of T or B cell lymphoma in dogs. This isotope control was used to mark the negative ...
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p38 MAPK (D13E1) XP® Rabbit mAb
(1)
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Cell Signaling Technology
January 30, 2017
I am studying molecular anomalies in signaling pathways leading to cancers and development of anti cancer immune therapies.
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p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb
(1)
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Cell Signaling Technology
January 27, 2017
I am studying molecular anomalies in signaling pathways leading to cancers and development of anti cancer immune therapies.
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ELISA Kit For IL-5
(1)
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January 27, 2017
Interleukin (IL)-5 is a well-known cytokine for the growth and survival factor for eosinophils and belongs to Th2 family cytokines.
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OK Staining Of CD3 On Human Lymphocytes By Flow Cytometry Using A Polyclonal Rabbit Anti-Human CD3 (IR503) Antibody
(1)
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Agilent Technologies
January 27, 2017
This particular antibody is meant for in vitro diagnostic use and comes ready to use for Dako Autostainer Link instruments. The specific ...
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K9 IP Experiment
(1)
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BioLegend
January 27, 2017
The aim of this experiment is to detect presence of T or B cell lymphoma in dogs. The antibody CD11b is used in gating the granulocytic ...
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CD184 (Cxcr4) Detection By Flow Cytometry
(1)
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BD Biosciences
January 27, 2017
The aim of this experiment was to detect the expression of CD185 on differentiated mouse embryonic stem cells. The overall objective of this...
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Dendrite Marker In Primary Mouse Neurons
(1)
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Abcam
January 27, 2017
Used this antibody as a dendrite marker to measure dendrite length. However, this antibody stained very weakly and we were unable to use ...
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Easy Cell Counting
(1)
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January 27, 2017
This is great for quickly counting cells in tissue culture. If you need to be very accurate, I would count twice as sometimes the numbers ...
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Decent Tubulin Antibody For Staining Dendrites
(1)
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January 26, 2017
Antibody stained dendrites well, but left background speckling.
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Isotype Control for K9 Immunophenotyping
(1)
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BioLegend
January 26, 2017
The aim of this experiment is to detect presence of T or B cell lymphoma in dogs. This isotope control was used to mark the negative ...
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A Goat Polyclonal Anti-Human CD3-Gamma Antibody (Clone: C-20) That Works Very Well In Flow Cytometry
(1)
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Santa Cruz Biotechnology, Inc.
January 26, 2017
Our lab works with tissues from several species and we are regularly in search of antibodies that work across several species and in ...
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Circulating Immune Complex (CIC) Detection And Quantification Using Quidel's MicroVue Enzyme Immunoassay Technology
(1)
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January 26, 2017
The MicroVue CIC EIA kit can be used to quantify CICs in small volumes of human serum or plasma. Each kit includes one 96-well plate that ...
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Routine Cell Culture Brightfield Analysis Using The Evox XL From ThermoFisher Scientific
(1)
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January 26, 2017
This system was used to analyzed in vitro cultures of differentiated pluripotent stem cells. The Evos XL allowed to conveniently observe, ...
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Fast RNA Extraction
(1)
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Zymo Research
January 26, 2017
This kit extracts RNA fast, but you get much more RNA and cleaner RNA with the regular trizol protocol.
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Mouse IL-13 ELISA
(1)
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January 26, 2017
I am studying molecular anomalies in signaling pathways leading to cancers and development of anti cancer immune therapies.
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A Collagen IV Antibody That Works For Drosophila
(1)
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Abcam
January 25, 2017
The aim of our research is visualizing and quantifying the extracelullar matrix proteins in the Drosophila heart. Specifically we note how ...
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Good Antibody Against STAT1
(1)
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BD Biosciences
January 25, 2017
We are working on extracellular vesicles and response pathways of different cell types due to diverse stimuli, especially by looking at the ...
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Good Antibody For Staining Active Caspase-3
(1)
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R&D Systems, a Bio-Techne Brand
January 25, 2017
We were looking for an antibody to stain active caspase 3 in cells and neurons and tested a few. This one worked consistently and well, ...
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Detects FLT3 Expression In Human Leukemic Cells
(1)
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January 25, 2017
The general aim of my research is to study cytokine receptor expression and signaling in human leukemic cells. We are measuring cytokine ...
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Great Way to Extract Small Amounts of RNA
(1)
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January 25, 2017
This kit is great to extract small amounts of RNA from primary mouse tissue.
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FITC Anti-Mouse CD193 Antibody For Flow Cytomeric Analyis
(1)
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BioLegend
January 25, 2017
CD193 is also known as C-C chemokine receptor type 3 (CCR3) and mainly belongs to G protein-coupled receptor. CD193 is highly expressed on ...
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Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (197G2) Rabbit mAb
(1)
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Cell Signaling Technology
January 25, 2017
I am studying molecular anomalies in signaling pathways leading to cancers and development of anti cancer immune therapies.
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Mouse IL-10 ELISA
(1)
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January 24, 2017
I am studying molecular anomalies in signaling pathways leading to cancers and development of anti cancer immune therapies.
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Super Easy Cloning
(1)
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January 24, 2017
The cold fusion cloning kit makes it super easy to clone any PCR product into almost any plasmid.
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Dendrite Marker: beta-3 Tubulin Antibody
(1)
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Thermo Fisher Scientific
January 24, 2017
This dendrite marker stains very well, but leaves background speckles in primary neuron cultures.
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Good Antibody For Staining G3BP In Cells And Tissues
(1)
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January 24, 2017
Antibody stains cells and tissues well. Staining presentation appears differently depending on what cells you are looking at. Cytoplasmic ...
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Good Antibody Against pSTAT1
(1)
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Cell Signaling Technology
January 24, 2017
We are working on extracellular vesicles and response pathways of different cell types due to diverse stimuli, especially by looking at the ...
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DP73 Digital Camera From Olympus
(1)
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Evident
January 24, 2017
This camera has been used in conjunction with the Olympus BX63 microscope to assess fixed bright field as well as fluorescent images. This ...
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Radial Immunodiffusion Plates To Measure Human IgG Subtypes (Including IgG1/2/3/4) In Serum, Plasma, Or Other Biological Fluids
(1)
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January 24, 2017
The IgG subtype RID kits from The Binding Site, ltd. are a simple, reliable benchtop assay for the quantification of individual IgG subtypes...
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Kits
Labels / Dyes / Stains
Ladders and Markers
Light Sources
Liquid Handling Equipment
Luminometer
Magnetic Beads
Magnets
Mass Spectrometer
Master Mixes
Media
Meter
Microplate Washer
Microscopes
Microtome
Mixer
PCR Equipment
Pipettor
Plate Reader
Precast Gels
Primary Cells
Proteins
Purification Equipment
Refrigerator / Freezer
Rocker
Sample Prep Equipment
Scanners
Services
Shaker
Slides
Software
Sonicator
Spectrometer
Sterilizer
Stirrer
Substrates
Transfection Equipment
Vacuum Manifolds
Vortexer
Water Bath
Applications
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Western Blot
Flow Cytometry
ELISA
Immunofluorescence
Immunoprecipitation
Immunocytochemistry
Immunohistochemistry
Block/Neutralize/Inhibit
Cell Isolation
Dot Blot
ChIP
Purification
Staining
Cell Culture
DNA Purification
PCR
RNA Analysis
RNA Purification
qPCR
Clean-up
Microscopy
Cloning
RT-PCR
cDNA Synthesis
Transfection
Electrophoresis
RT-qPCR
Cell Counting
Protein Purification
Next Gen Sequencing (NGS)
Library Construction
Genotyping
Gel Imaging
Protein Assay
Reporter Assay
Cell Lysis
Multiplex
Gel Staining
RNA-Seq
Live Cell Imaging
Cell Differentiation
Ligation
Concentration
HPLC
Sequencing
Chromatography
Mass Spectrometry
Labeling
Microarray
Mutagenesis
FISH
CRISPR
TUNEL Assay
In Vivo Imaging
EMSA
Cell Fractionation
Immunoassay
In Vitro Transcription
ChIP-Seq
Northern Blot
3D Imaging
RNAi
ELISPOT
PCR Cloning
Particle Analysis
Southern Blot
Protein Modification
DNA Analysis
Expression
Cell-Based Assay
Gene Editing
In Vivo Testing
Protein Labeling
Other
Species
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Human
Mouse
Rat
Non-Human Primate
Rabbit
Sheep
Hamster
Chicken
Bovine
Canine
Porcine
Horse
Monkey
C. elegans/Worm
Insect
Amoeba/Protozoa
Bacteria/Archaea
Arabidopsis
Yeast/Fungi
Squirrel
Fish
Virus
Other Invertebrate
Xenopus/Amphibian
Drosophila
Goat
Rhesus
Tissue
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Adipose
Aorta
Artery
Bladder
Blood
Blood Vessel
Bone
Bone Marrow
Brain
Breast
Bronchus
Cerebral Cortex
Colon
Cornea
Coronary Artery
Dermis
Duodenum
Ear
Embryo
Endometrium
Epidermis
Eye
Foreskin
Gingiva
Hair
Heart
Hippocampus
Hypothalamus
In Vivo
Intestine
Kidney
Large Intestine
Liver
Lung
Lymph Node
Mammary Gland
Mouth
Muscle
Ovary
Pancreas
Peripheral Blood
Peritoneum
Placenta
Prostate
Rectum
Retina
Skeletal Muscle
Skin
Small Intestine
Spinal Cord
Spleen
Stomach
Striatum
Synovium
Tail
Teeth
Testis
Thymus
Thyroid Gland
Tonsil
Trachea
Umbilical Cord
Umbilical Vein
Uterus
Vein
Cell Type
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Adipocyte
Astrocyte
B Cell
CD8+ T Cell
Competent Cells
Dendritic Cell
Embryonic Stem Cell
Endothelial Cell
Eosinophil
Epithelial Cell
Fibroblast
Glial Cell
Granulocyte
Helper T Cell
Hematopoietic Stem Cell
Hepatocyte
HUVEC
Induced Pluripotent Stem Cell
Keratinocyte
Leukocytes
Lymphocyte
Macrophage
Mesenchymal Stem Cell
Microglia
Microvascular Endothelial Cell
Monocyte
Myoblasts
Myocyte
Neuron
Neutrophil
NK Cell
NKT Cell
Oligodendrocytes
Osteoclast
PBMC
Platelets
Progenitor Cell
Red Blood Cell
Regulatory T Cells
Skeletal Muscle Cell
Smooth Muscle Cell
Splenocyte
Stem Cell
Stromal Cell
Synoviocyte
T Cell
Thymocytes
Tumor Cells
Cell Line
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HeLa
PC3 (PC-3)
HEK293
HEK293T (HEK-293T)
CHO
Jurkat
3T3
Caco-2
HepG2
THP-1
U2OS
PC12 (PC-12)
HL60 (HL-60)
A375 (A-375)
MOLT4 (MOLT-4)
MCF-7
A431
MDA-MB-231
A549
N2a
Daudi
HCT-116
Raji
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