GAPDH Antibody

Physiology and Biophysics
Albert Einstein College of Medicine
Graduate student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Fitzgerald

Product Name:

GAPDH antibody

Catalog Number:

10R-G109a

The general aim was to quantify levels of various endogenous proteins after treatment with siRNA. The goal was to use GAPDH as a loading control to allow for quantification and comparison between samples. GAPDH is widely used in the field as a loading control. This antibody has been reported in the literature. After testing, we found that it performs very well.

Experimental Design and Results Summary

Applications

Western Blot

Sample

HUVEC lysate

Primary Incubation

1:25000, 1 hour

Blocking Agent

5% milk

Secondary Incubation

1:5000, 1 hour

Tertiary Incubation

NA

Detection

ChemiDoc™ Touch Imaging System

Results Summary

Western blot analysis to detect endogenous levels of GAPDH in HUVEC samples.

DOI or PMID #

NA

Additional Notes

NA

Related Categories

Image Gallery

Summary

The Good

Works great, relatively quick incubation time

The Bad

None

The Bottom Line

Great antibody for GAPDH loading control

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