R&D Systems
Human SOX9 Antibody
AF3075
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The aim of this experiment was to detect the expression of Sox9 in fixed mouse embryonic stem cells. The goal was to observe Sox9 positive-stained cells. The specific reason for selecting this antibody was that it was recommended by colleagues.
Immunofluorescence
Fixed mouse embryonic stem cells
1:100 dilution, either over night at 4 degrees C or 1 hr at room temperature.
Donkey serum
Invitrogen Alexa 647-donkey anti-goat
Not applicable.
Immunofluorescence- Microscopy
Sox9-positive cells were detected in the fixed mouse embryonic stem cells.
Ease of use
Very high background.
Look for alternative Sox9 antibody for mouse cells.