Easy, Fast, High Yield Midiprep

UCSF
Urology
Grad Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Zymo

Product Name:

ZymoPURE™ Plasmid Midiprep Kit

Catalog Number:

D4200

I’m studying regulatory sequences in embryonic stem cells. This product was used to clone reporter plasmids to test potential regulatory sequences. I choose this kit after receiving a trial kit from a vendor or neighboring lab and having them work well. This is probably the fastest and easiest midiprep kit.

Experimental Design and Results Summary

Application

Purify plasmids

Starting Material

Bacteria transformed with a plasmid

Protocol Overview

Lyse and neutralize bacteria, filter debris, add precipitation buffer and flow through columns on a centrifuge.

Tips

Don't overgrow your bacteria or add too much to the columns

Results Summary

Consistently get high concentrations of clean plasmid

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Super fast and easy

The Bad

None

The Bottom Line

I would highly recommend for any plasmid prep.

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