FITC Anti-Mouse CD193 Antibody For Flow Cytomeric Analyis

Pulmonary disease
Tulane University
Post Doc

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Biolegend

Product Name:

CD193

Catalog Number:

144509

CD193 is also known as C-C chemokine receptor type 3 (CCR3) and mainly belongs to G protein-coupled receptor. CD193 is highly expressed on eosinophils.CD193 also binds a variety of chemokines, such as eotaxins, MCP-3, MCP-4 and RANTES. Here our interest was to detect the eosinophils in the spleen of mouse after the allergen challegne. Therefore, FITC-anti-mouse CD193 antibody was used to detect eosinophils.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse spleen

Primary Incubation

2ug/1million cells for 30 min

Blocking Agent

0.01% BSA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

FL-1-FITC filter

Results Summary

FITC-anti-mouse CD193 antibody was used to detect eosinophils form mouse spleen and we have detected the presence of eosinophils and results were as expected.

DOI or PMID #

NA

Additional Notes

NA

Related Categories

Image Gallery

Summary

The Good

Only 30 min of incubation period is required to stained the cells

The Bad

NA

The Bottom Line

Overall CD193 antibody is work very well in mouse spleen when used by Flowcytometry.

Share your experience with other scientists. Write a Review! »

Join the discussion