Mouse IL-10 ELISA

Harvard
Harvard Medical School
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

eBioscience

Product Name:

Mouse IL-10 ELISA Ready-SET-Go! (2nd Generation)

Catalog Number:

88-7105-86

I am studying molecular anomalies in signaling pathways leading to cancers and development of anti cancer immune therapies.

Experimental Design and Results Summary

Application

IL-10 detection in biological samples

Starting Material

Mouse tissue homogenates

Protocol Overview

Overnight incubation at 4 degree with capture antibodyBlocking with 200 µL/well of 1X ELISA/ELISPOT DiluentOvernight incubation assay supernatant at different dilutions1 hour incubation with detection antibody at room temperatureELISA-liquid TMB substrate based development

Tips

Standardize sample dilution first as per the kit detection range

Results Summary

High levels of IL-10 were detected in mice tissue homogenates.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Nice and efficient IL-10 detection

The Bad

None

The Bottom Line

Nice IL-10 detection ELISA kit, gives reliable results when used within expiry date of the kit.

Join the discussion