Kristen Valentine

Graduate Student
University of California, Merced

Articles by Kristen Valentine

  • Antibody User Review

    Decent CD4 Antibody Staining

    February 14, 2018
    This antibody titrates out well but not as well as other fluorophores of the same clone. It can be used in a variety of ... read more
  • Antibody User Review

    Bright And Clean CD45R Antibody

    February 08, 2018
    This antibody works well to stain CD45R on B cells with little to no optimization and at a decent titration. Other fluorophores ... read more
  • Assay Kit/Reagent User Review

    Bright Fixable Live/Dead Marker

    October 18, 2017
    This stain can be detected on a red laser with a 780/60 bandpass filter. It fits well in complex flow cytometry staining panels ... read more
  • Antibody User Review

    Antibody to Detect Bcl-6

    October 06, 2017
    We use Bcl-6 to investigate CD4 T follicular helper cells. Invitrogen suggests to use this antibody at a fairly dilute ... read more
  • Antibody User Review

    Great CD8a Antibody

    October 05, 2017
    This antibody works well at a great titration to separate out CD8 T cells. We use this antibody at 1:1600 and always have a ... read more
  • Antibody User Review

    Bright and Clean TCR beta Antibody

    October 04, 2017
    We use this antibody to identify T cells during autoimmune disease. This antibody titrates well for a bright and distinct antibody... read more
  • Assay Kit/Reagent User Review

    Easy To Use Ethidium Bromide Replacement

    October 03, 2017
    SYBR Safe is a bright easy to use replacement for ethidium promode in DNA agarose gels. It requires little to no optimization and ... read more
  • Assay Kit/Reagent User Review

    Reliable Way To Fix Cells For Transcription Factor Staining

    September 27, 2017
    This kit is advertised for staining FoxP3 in T regulatory cells but it works well for all transcription factors and some cytokines... read more
  • Assay Kit/Reagent User Review

    Highly Efficient And Easy To Use Lysate Tool

    September 21, 2017
    We utilize Qiagen RNeasy Micro Kit to do most of our RNA extractions. Originally we had lysed lymphocytes using a 27g syringe. To ... read more
  • Assay Kit/Reagent User Review

    Great for Density Gradient Separation

    September 20, 2017
    We use this reagent to separate white blood cells from red blood cells using a ficoll density of 1.084. You can purchase different... read more
  • Antibody User Review

    Consistent TCR beta Antibody

    September 19, 2017
    This antibody can be reliably titrated and stains consistently bright. We use this antibody to investigate T cells during ... read more
  • Equipment User Review

    Lancets for Submandibular Blood Collection

    September 18, 2017
    These lancets work well for submandibular blood collect. They come in a variety of sizes (3, 4, 5, 5.5, 6, 7, and 8mm) that can ... read more
  • Assay Kit/Reagent User Review

    Reliable TMB Substrate Kit

    September 14, 2017
    This is an easy to use TMB substrate kit for a number of applications. We use this TMB for use in sandwich ELISAs. Provided your ... read more
  • Antibody User Review

    Bright ICOS Antibody Staining

    September 12, 2017
    This antibody, when properly optimized gives bright and distinct signal. The conjugated APC maintains its stability for over two ... read more
  • Equipment User Review

    Functional Benchtop Orbital Shaker

    September 11, 2017
    This orbital shaker fits nicely on the Benchtop surface. It is programmable up to 300rpm for 100 hours. We use this orbital shaker... read more
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