Bright ICOS Antibody Staining

September 12, 2017

Molecular and Cell Biology
University of California, Merced
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen Antibodies

Product Name:

CD278 (ICOS) Monoclonal Antibody (C398.4A), APC, eBioscience™

Catalog Number:

17-9949-80

This antibody, when properly optimized gives bright and distinct signal. The conjugated APC maintains its stability for over two weeks when fixed. We utilize this antibody to investigate T cells in autoimmune disease and find it to be bright and reliable regardless of the size of the cell population.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine T lymphocytes

Primary Incubation

Dilute 1:200 for 30 minutes at 4 degrees Celcius

Blocking Agent

FC Block

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD LSR II or BD FACS Aria II

Results Summary

Fixed or unfixed, this antibody maintains robust signal for a variety of applications. On some populations ICOS can appear as a shoulder population but it can be easily identified in as a histogram with B cells as a generally reliable negative control.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Bright stain that can be titrated

The Bad

Be careful when titrating but for some applications it can be used at a greater dilution than 1:200.

The Bottom Line

This works well for staining ICOS and can maintain robust color for up to 2 weeks on fixed cells.

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