Consistent TCR beta Antibody

Molecular and Cell Biology
University of California, Merced
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen Antibodies

Product Name:

TCR beta Monoclonal Antibody (H57-597), FITC, eBioscience™

Catalog Number:

11-5961-82

This antibody can be reliably titrated and stains consistently bright. We use this antibody to investigate T cells during autoimmune disease. This antibody works well for a variety of applications, including cell sorting and fixation.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine T lymphocytes

Primary Incubation

Dilute 1:400 for 30 minutes at 4 degrees Celcius

Blocking Agent

FC Block

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD LSR II or BD FACS Aria II

Results Summary

This antibody will consistently show two distinct population in T cell subsets. It can be titrated further than 1:400 for some applications.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Bright stain that can be titrated

The Bad

Other fluorophore conjugates titrate out further than this one.

The Bottom Line

It stains TCR beta on T cell populations with consistent bright color once it is titrated.

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