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Author Profile
Andrea Wong
Postdoctoral research fellow
Bloodworks Northwest
Articles by Andrea Wong
Antibody User Review
Review of CD4 APC-eFluor 780
March 03, 2016
To design a 10 color panel, I needed to find an antibody that will perform well for this relatively dim fluorochrome as APC-...
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Antibody User Review
Review of KLRG-1 Brilliant Violet 421
February 25, 2016
KLRG-1 is expressed on memory T cells and NK cells and has been reported to exhibit inhibitory functions in these cell types. In ...
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Antibody User Review
Use of CXCR5-PE to Identify T Follicular Helper Cells by Flow Cytometry
February 24, 2016
The goal of this experiment was to identify T follicular helper cells (Tfh) by flow cytometry using canonical markers such as ...
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Antibody User Review
Review of Anti-Mouse V alpha 2 TCR PerCP-eFluor® 710
February 23, 2016
This antibody against the Va2 TCR when used in combination with Vb5 TCR is routinely used to identify OT-II CD4 T cells, a ...
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Antibody User Review
Review of Anti-Mouse CD45 APC-eFluor 780
February 22, 2016
This antibody reacts with all isoforms of mouse CD45 and has been useful when I needed to identify hematopoietic cells in whole ...
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Antibody User Review
Excellent CD69 Antibody
October 15, 2015
This antibody was to used to identify recently activated T cells and it worked very well.
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Antibody User Review
Good Antibody
October 09, 2015
We routinely use this antibody in our 8-10 color multi parameter flow cytometry analysis and the CD8a antibody conjugated to APC-...
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Antibody User Review
Review of Anti-Ki67 eFluor450
October 06, 2015
We wanted to examine if our TCR transgenic CD4 T cells were proliferating in response to an autoantigen straight out of an animal ...
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Antibody User Review
Review of CD4+ T Cell Isolation Kit via Negative Selection
October 05, 2015
We routinely perform adoptive transfer of CD4 T cells into B6.Thy1.1 recipients and analyze their proliferation in response to ...
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Antibody User Review
Decent Antibody
October 02, 2015
The Alexa700 conjugated anti-mouse CD44 antibody is a decent antibody for this super dim fluorochrome.
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Antibody User Review
Decent Viability Dye
September 28, 2015
This dye was used to discriminate between live and dead cells in experiments that needed fixation and permeabilization for ...
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Antibody User Review
Good Antibody for This Dim Fluorochrome
September 25, 2015
The APC-eFluor780/APC-Cy7 fluorochrome is known to be a pretty dim color so when choosing an antibody for this channel, we aim to ...
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Antibody User Review
Good Alternate Fluorochrome for the AmCyan Channel
September 23, 2015
I tried the anti-TCRbeta (clone H57) antibody conjugated to Brilliant Violet 510 when designing a multicolor panel to identify ...
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Antibody User Review
A good antibody for platelet identification
June 16, 2015
This antibody is generally used to identify platelets in the whole blood. In combination with the forward scatter/side scatter, ...
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Antibody User Review
Brilliant fluorochrome for a dim marker
June 15, 2015
This antibody was used in a staining panel to identify Tregs in the thymus and the spleen. The CD25-Brilliant Violet 421 antibody ...
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Efficient and Cost Friendly SYBR Green Mastermix for qPCR
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I study the transcription of endogenous genes regulated by transcription factors, and it's crucial to quantify their expression levels accurately. To achieve ...
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