eBioscience
Anti-Mouse V alpha 2 TCR PerCP-eFluor® 710
46-5812-80
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This antibody against the Va2 TCR, when used in combination with Vb5 TCR, is routinely used to identify OT-II CD4 T cells, a transgenic TCR specific for the OVA peptide presented in the context of I-Ab. This antibody works very well and gives a great separation between positive and background.
Flow Cytometry
Splenocytes
30 mins +4c.
Anti-CD16/32
NA
LSR II
In the OT-II mouse, >90% of the CD4 T cells are Va2+Vb5+. Similar staining panel performed in B6 mice resulted in less than 0.5% cells positive for both markers. This is a very bright fluorochrome and can result in a significant spillover into the PeCY7 channel. Might need to adjust the voltage accordingly.
None
Great antibody
Can result in significant spillover into the PeCY7 channel.
Highly recommend.