Mithun Khattar

Research Fellow

Dana Farber Cancer Institute

A Good Antibody For L-Selectin Staining On Lymphocytes
November 22, 2016
This review is about L-selectin staining on mouse lymphocytes. In the mouse, L-selectin is detected on most thymocytes, with the ... read more
Activation marker for T cells in mice
November 16, 2016
CD44 is an activation/memory marker that gets upregulated on T cells and B cells. CD44 is an adhesion molecule involved in ... read more
Brilliant Violet Antibody for CD4
November 15, 2016
CD4 is a very common marker in lymphocyte staining panels. Since its a lineage marker, I wanted to try staining it using uncommon ... read more
Not My Best CD4 Staining
November 14, 2016
I used this antibody as a compensation control for a high bandwidth flow cytometry experiment. Although, it served the purpose i ... read more
Good Staining With A Few Catches
November 11, 2016
The staining worked fine for detecting CD4 positive cells. It wasn't as bright as I had expected it to be based on prior ... read more
Really Good Staining On A Weak Fluorophore
October 31, 2016
I have used anti-CD4 Ab in pretty much all fluorescent colors available, as it is a popular marker in my analyses. Perhaps, I can ... read more
APC-Cy7 Labeled Anti-Mouse CD4 Antibody
August 08, 2016
CD4, also known as L3T4 or T4 is expressed on most thymocytes and a subset of T cells, and weakly on macrophages and dendritic ... read more
Good Antibody If You Can Compensate Well
August 03, 2016
CD4 is expressed on a subset of T cells in thymus, spleen and peripheral lymphoid organs. Although the expression of CD4 might ... read more
A Good Brilliant Violet Antibody for CD4
July 27, 2016
Since CD4 is a lineage marker, it doesn't really require a bright fluorophore like BV785 for detection. However, I used this ... read more
PE Conjugated Anti-Mouse CD4 Antibody
July 22, 2016
PE or phyco-erythrin is my favorite fluorophore and I used it for detecting CD4 T cells within mouse splenocytes. Thus far, this ... read more
Pretty Decent CD4 Staining Even With A Dim Fluorophore
July 18, 2016
This antibody was used for staining CD4+ cells within mouse splenocytes. Even though PERCP-Cy5.5 is a really dim fluorophore, ... read more
Detect Antigen Presentation Of OVA On MHC-1 Using Flow Cytometry
July 15, 2016
This antibody specifically reacts with the ovalbumin-derived peptide SIINFEKL bound to H-2Kb of MHC class I (which is present in ... read more
CD4 Expression On The PE-Cy7 Channel
July 13, 2016
This antibody was used as a compensation control for the PE-Cy7 channel. Although it worked it just fine for my intended purpose, ... read more
Very Bright Fluorescent Detection Of CD4
July 12, 2016
Anti-CD4 antibody was used for staining mouse splenocytes. CD4 is expressed by a majority of thymocytes, subpopulation of mature T... read more
A Good Antibody For Measuring CD4 Expression
July 08, 2016
CD4 is expressed on a subset of T cells called helper T cells and is considered as a lineage marker. I used this antibody as a ... read more

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Excellent Astrocyte Labeling with Low Background in Mouse Hippocampus

Ksenia Novototskaya

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We used this antibody to immunofluorescently label astrocytes in the mouse dorsal hippocampus following astrocyte-specific MOR knockdown. The signal was bright,... read more

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