Biolegend
PE anti-mouse H-2Kb bound to SIINFEKL
141604
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This antibody specifically reacts with the ovalbumin-derived peptide SIINFEKL bound to H-2Kb of MHC class I (which is present in C57BL/6 mice), but not with unbound H-2Kb or H-2Kb bound with an irrelevant peptide. I used this antibody to stain a transgenic cell line that expresses mouse Kb. The assay worked perfectly and specific expression of SIINFEKL loaded cells was detected using this antibody.
Flow Cytometry
K562 transgenic cells
25 minutes on ice
CD16/32 antibody for blocking Fc receptors
None
Flow cytometry on PE channel
Specific staining was observed only when cells were pulsed with 1uM SIINFEKL peptide. Non-pulsed cells or irrelevant cells pulsed with SIINFEKL did not show any expression.
Very good staining
A bit expensive
Although a bit expensive, this antibody facilitates a really good assay to detect antigen presentation.