eBioscience
AF488 anti-mouse CD4 (GK1.5)
53-0041-82
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The staining worked fine for detecting CD4 positive cells. It wasn't as bright as I had expected it to be based on prior experiences with AF488, but it was fine for the purpose as CD4 is a lineage marker. One word of caution is that the GK1.4 clone is known to deplete CD4 T cells in vivo, so it may not be the best antibody for cell sorting and adoptive transfer based experiments.
Flow Cytometry
Mouse splenocytes
25 minutes on ice
CD16/32 antibody for blocking Fc receptors
None
Flow cytometry on FITC channel
The staining wasn't too bright, but relevant and specific.
N/A
Easy to use
Weak staining, depletion in vivo
Works just fine for analysis based applications