Really Good Staining On A Weak Fluorophore

Cancer Immunology & AIDS
Dana Farber Cancer Institute
Research Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

BV650 anti-mouse CD4 (RM4-5)

Catalog Number:

100548

I have used anti-CD4 Ab in pretty much all fluorescent colors available, as it is a popular marker in my analyses. Perhaps, I can say that this is my favorite Ab when performing multi-parametric analysis involving more than 10 colors. The staining is relevant with minimal background and the expression level is perfect for compensation with adjacent fluorophores.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

25 minutes on ice

Blocking Agent

CD16/32 antibody for blocking Fc receptors

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry on BV650 channel

Results Summary

The staining is relevant with minimal background shift in the negative population.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Bright and relevant staining

The Bad

These colors are little expensive

The Bottom Line

Great antibody for the price

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