Biolegend
BV711 anti-mouse CD4
100557
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CD4 is expressed on a subset of T cells in thymus, spleen and peripheral lymphoid organs. Although the expression of CD4 might change during an ongoing immune responses, it is considered as a lineage marker i.e., CD4+ T cells always express it at some level. I used this antibody to detect CD4+ T cells in mouse splenocytes. The staining was relevant and background was minimal. However, BV711 is very close to BV786 which made it difficult to compensate when both colors were used in the panel.
Flow Cytometry
Mouse splenocytes
25 minutes on ice
CD16/32 antibody for blocking Fc receptors
None
Flow cytometry on BV711 channel
The staining is almost perfect. Among the BV colors, this antibody performed the best in terms of highest fluorescence with least background.
Perfect staining with >4 log shift
Bleeds into BV786 channel
The best you can get on a violet laser.