Biolegend
BV785 anti-mouse CD4
100552
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Since CD4 is a lineage marker, it doesn't really require a bright fluorophore like BV785 for detection. However, I used this antibody for preparing compensation controls for this channel. I was a bit skeptical due to lower expression of CD4 compared to some other targets, but I noticed a very bright stain using this fluorophore. There was >4 log shift fluorescence with minimal background.
Flow Cytometry
Mouse splenocytes
25 minutes on ice
CD16/32 antibody for blocking Fc receptors
None
Flow cytometry on BV786 channel
The results were flawless. There was no background in the negative population and the positive cells were very bright.
Bright and relevant staining
Compensation issues with other BV colors
It is an optimal antibody for both detection and measuring expression kinetics of CD4