eBioscience
PERCP-Cy5.5 anti-mouse CD4
45-0042-82
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Browse Similar Products: CD4 Flow Cytometry Antibodies
This antibody was used for staining CD4+ cells within mouse splenocytes. Even though PERCP-Cy5.5 is a really dim fluorophore, there was a distinct separation between the positive and negative cells. Apart from the small background stain in negative cells, the staining was relevant and succinct for the target marker.
Flow Cytometry
Mouse splenocytes
25 minutes on ice
CD16/32 antibody for blocking Fc receptors
None
Flow cytometry on PERCP-Cy5.5
Although the level of fluorescence was low, specific expression of CD4+ cells was observed. A small level of background was observed, but it was better than some of the other antibodies from Ebioscience
Specific staining and low background
Dim fluorescence
For a lineage marker, this antibody works great.