eBioscience
APC anti-mouse CD4
17-0042-82
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CD4 is expressed on a subset of T cells called helper T cells and is considered as a lineage marker. I used this antibody as a compensation control for the APC channel. The expression was specific and fluorescence was good. Except for a non-specific shift in the negative population, the antibody worked perfectly fine.
Flow Cytometry
Mouse splenocytes
25 minutes on ice
CD16/32 antibody for blocking Fc receptors
None
Flow cytometry
The fluorescence was good and specific staining was observed. A small shift in the negative population was also observed which may affect experiments looking at CD4 expression changes over time.
Easy to use
eBioscience antibodies usually have a background fluorescence
A great antibody if APC is the only available channel