Biolegend
BV421 anti-mouse CD4
100563
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CD4 is a very common marker in lymphocyte staining panels. Since its a lineage marker, I wanted to try staining it using uncommon fluorophores, especially on the brilliant violet laser. Among all the ones tested in the BV spectrum, this performed the best. Staining was succinct, bright and highly specific. A highly recommended antibody.
Flow Cytometry
Mouse splenocytes
25 minutes on ice
CD16/32 antibody for blocking Fc receptors
None
Flow cytometry on BV421 channel
Very bright (3 log shift) and specific staining was observed with this antibody.
N/A
Bright and specific staining
Expensive and hard to compensate with adjacent fluorophores like BV510
Highly recommended for the price.