Biolegend
BV-785 anti-mouse CD44
103059
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CD44 is an activation/memory marker that gets upregulated on T cells and B cells. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation. I wanted to test T cell activation using this antibody. Perhaps the staining worked fine. The expression levels of CD44 were in a broad range, which is consistent with published literature.
Flow Cytometry
Mouse splenocytes
25 minutes on ice
CD16/32 antibody for blocking Fc receptors
None
Flow cytometry on BV786 channel
The staining worked as expected. There were cells with low, mid and high expression, consistent with published data.
N/A
Bright and specific staining
Difficult to compensate due to braod expression range
Recommended with unique fluorophores