Kristi Anderson

Graduate student

University of Nebraska Medical Center

Western Blot Stripping Buffer Works Great and Can Re-Use
August 03, 2015
For Western blot analysis, we strip the membranes so we can identify two proteins that are at very similar molecular weights. read more
Easy for Routine Genotyping
July 31, 2015
We genotype a lot of mice and this works well with agarose gels. Bands are easily detectable and it's easy to increase or decrease... read more
Immunohistochemical staining of SNpc
April 27, 2015
Used for staining of substantia nigra following MPTP intoxication to quantitate surviving dopaminergic neurons. Following ... read more
Makes marking band sizes easy
April 23, 2015
This was used as the ladder when running Western blots to mark molecular weights on the gel. Used with 4-15% gels and transferred ... read more
Easy RNA clean up
April 16, 2015
Used this kit to clean up RNA from several sources: tissue, primary neurons, cell lines. The end goal is to run qPCR on these ... read more
Cost effective and easy
April 15, 2015
Used this kit to convert to cDNA from primary neurons, cell lines, and DNA from tissues with the ultimate goal of running qPCR on ... read more
Easy to use for protein quantification
April 08, 2015
This product was used in Western blot assays looking at various proteins in the brain homogenate of MPTP-intoxicated animals. ... read more
Good but not great for staining tissues
March 27, 2015
Following MPTP, we wanted to quantify the total number of surviving neurons in the SNpc using an antibody specific for MAP2. Fixed... read more
Not useful for looking at brain homogenate
March 26, 2015
The phosphorylation of TH at the different serine residues was evaluated in MPTP-intoxicated mice. Following MPTP, brain regions ... read more
Easy to use for immunofluorescence
March 25, 2015
This antibody is used to evaluate the number of GAD67+ neurons in various brain regions of mice that have been intoxicated with ... read more
Works well for brain homogenate (mouse)
March 25, 2015
This antibody was used to look at changes in tyrosine hydroxylase phosphorylation at serine 31 in mice following MPTP. 7 days post... read more
Used for evaluation of whole brain homogenate
March 24, 2015
This antibody was used to evaluate the effects that MPTP can have on the phosphorylation status of tyrosine hydroxylase at ... read more
So happy I found this beta-actin antibody
March 03, 2015
Doing western blots became a lot easier using this pre-conjugated beta actin antibody. I use it on brain homogenate from MPTP-... read more
Immunofluroescent staining fixed tissues
February 27, 2015
In mouse models of Parkinson's Disease we use multiple primary antibodies to look at neuron populations following addition of MPTP... read more
Secondary antibody to view neurons within midbrain
February 26, 2015
This antibody is used in conjunction with an anti-tyrosine hydroxylase antibody to view dopaminergic neurons in the SN in the MPTP... read more

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Excellent Astrocyte Labeling with Low Background in Mouse Hippocampus

Ksenia Novototskaya

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We used this antibody to immunofluorescently label astrocytes in the mouse dorsal hippocampus following astrocyte-specific MOR knockdown. The signal was bright,... read more

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