Cell Signaling Technology
Phospho-tyrosine hydroxylase (ser40)
2791
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The phosphorylation of TH at the different serine residues was evaluated in MPTP-intoxicated mice. Following MPTP, brain regions were isolated, homogenized, purified, and run on Western blots. Membranes were probed for phospho-TH (ser40) and expression quantified using ImageJ.
Western Blot
Brain homogenate
1:500 in 5% BSA/TBST. Overnight at 4 degrees
5% BSA/TBST
1:10,000 HRP-conjugated secondary, 60 minutes at room temp
NA
Femto Chemiluminescent
There was a fairly light band at ~55 kDa (where the protein should be detected) but there were also bands and multiple other molecular weights. This could be due to using whole brain homogenate instead of PC-12 cells. I re-ran several gels to attempt to get better results with no luck.
None
Seems like it would work well on PC-12 cells
Multiple bands at incorrect molecular weights. Did work well on brain homogenate.
Didn't get any useable data with this antibody. Need to find a different one.