Cell Signaling Technology
Phospho-tyrosine hydroxylase (ser31)
3370
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This antibody was used to look at changes in tyrosine hydroxylase phosphorylation at serine 31 in mice following MPTP. Seven days post-MPTP brain regions of interest were isolated, homogenized, and purified. Brain samples were evaluated via Western blot.
Western Blot
Brain homogenate
1:500 in 5% BSA/TBST. Overnight at 4 degrees
5% BSA/TBST
1:10,000 HRP-conjugated secondary, 60 minutes at room temp
NA
Femto Chemiluminescent
Using Western blot, we were able to easily detect levels of phospho-TH (ser31) in our brain samples. The binding for this antibody seemed to be very specific and at the correct molecular weight (around 55 kDa).
Definitely use 5% BSA (dry milk DOES NOT work)
Very nice signal
Need to use relatively high concentration. Can't use dry milk.
If you are looking at phospho-TH in brain homogenate, this antibody works great.