Vector Laboratories
Biotinylated Goat Anti-Rabbit IgG Antibody
BA-1000
Browse Similar Products: Biotinylated IgG Antibodies
Used for staining of substantia nigra following MPTP intoxication to quantitate surviving dopaminergic neurons. Following incubation of fixed tissues with anti-tyrosine hydroxylase antibody the biotinylated secondary antibody is used to visualize neurons. Tissues are counter-stained with thionin.
Immunohistochemistry
Fixed brain tissue
Anti-TH (1:2000) 48 hrs at 4 degrees
5% normal goat serum
1:400, 1 hour at RT in 2% NGS
NA
DAB
Used for quantifying both cell bodies within the SN and termini within the striatum is easy. Optimize the color development step and counter-staining to get the best results so you can clearly see individual bodies and their nuclei. Can easily quantitate neuron numbers with stereology and the striatal density using ImageJ
None
Consistent results
We've used this antibody for years. Have never had a problem with different lots not working or needing to re-titrate antibody