Spring Bioscience
Anti Glutamic acid decarboxylase
E10264
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Browse Similar Products: Glutamic Acid Decarboxylase 67 Antibodies
This antibody is used to evaluate the number of GAD67+ neurons in various brain regions of mice that have been intoxicated with MPTP or a variety of drugs. Using standard stereological methods it was easy to quantify the neurons using a 40x objective.
Immunofluorescence
Fixed brain tissue
1:500 in 2% normal donkey serum/TBS
5% NDS/TBS
1:2000 in 2% NDS/TBS, 1 hour, room temperature
NA
Texas Red fluroescence
At 40x, it was easy to identify GAD67+ neurons with little background fluorescence or non-specific staining. At lower magnifications (4x) it was difficult to identify the regions where GAD67+ neurons were located.
None
Good staining. Can order 1 mL for relatively cheap
Need to use high objective
This antibody works well for fixed brain tissue sampels.