EMD Millipore
Anti-tyrosine hydroxylase phospho Ser19
AB5425
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This antibody was used to evaluate the effects that MPTP can have on the phosphorylation status of tyrosine hydroxylase at serine19. Following MPTP, midbrain and striatal regions of the brain were isolated, homogenized, and purified before being loaded onto a gel and transferred to PVDF membrane. Membranes were incubated in primary phospho Ser19 antibody overnight at 4 degrees followed by and HRP-conjugated secondary antibody.
Western Blot
Whole brain homogenate
1:1000 in 5% dry milk/PBST. Overnight at 4 degrees.
5% dry milk/PBST for 45 - 60 minutes at room temperature
1:10,000 HRP-conjugated secondary, 60 minutes at room temp
NA
Femto Chemiluminescent
Data sheet states antibody recognizes protein of 60 kDa which is where I found a dark band. However, there were many other bands at different molecular weights. Could be non-specific binding or differences between performing Western blot on PC-12 cells and brain homogenate. Even though the data sheet states this antibody has only been tested on rats I used it on mice and results were good.
None
Good detection at 60 kDa
Also detected bands at other molecular weights
This antibody works well for detecting phospho Ser19 from mouse brain.