Anti-EGFR antibodies are used in the immunodetection of the protein epidermal growth factor receptor. In humans, the canonical protein has a reported length of 1210 amino acid residues and a mass of 134.3 kDa. Its subcellular localization is in the nucleus, ER, Golgi, and cell membrane. Up to 4 different isoforms have been reported for this protein. It is ubiquitously expressed across many tissue types. A member of the Tyr protein kinase protein family, EGFR is a known receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses.
The EGFR marker can be used to identify Cytotrophoblasts (CTB) and Syncytiotrophoblasts (STB).* Synonyms for this target antigen include ERBB1, ERRP, HER1, NISBD2, PIG61, mENA, EGFR vIII, and ERBB. EGFR gene orthologs have been reported in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species. Over 7300 citations in the literature describe the use of EGFR antibodies in research. Western Blot is a widely used application for these antibodies. In addition, Immunohistochemistry is also a common application.
*HuBMAP Human Reference Atlas v1.4