Fig 2: FGF10-dependent EGFR_T693 phosphorylation requires FGFR and ERK AList of compounds and their targets.B–DLysates from BT549 transfected with HA-FGFR1c, 2b, or 4 and stimulated for 8 or 40 min with Enkamin-E, FGF10 or FGF1, respectively, followed by treatment with either DMSO or the FGFR inhibitor PD173074 (B); BT549 transfected with HA-FGFR2b or HA-FGFR2b_Y656F/Y657F and stimulated with FGF10 for 40 min. (C); T47D treated with DMSO, PD173074 or the EGFR inhibitor AG1478 and stimulated or not with either FGF10 or TGFa for 0, 8 and 40 min. (D) were immunoblotted with the indicated antibodies.EProtein sequences surrounding T693 (based on UniProt P00533) of members of the ErbB family were aligned using CLUSTAL O (version 1.2.4). The red box indicates that the amino acid T is followed by a conserved proline. Asterisks below the sequences indicate identical amino acid residues; double dots indicate conserved amino acid residues; single dots indicate semi-conserved substitutions.F, GLysates from T47D or BT20 treated with DMSO, PD173074, AG1478 or the MEK inhibitors U0126 and MEK162 and stimulated or not with either FGF10 or TGFa (F); T47D or BT20 treated with DMSO, or the p38 inhibitor BMS582949 and stimulated or not with either FGF10 or TGFa (G) were immunoblotted with the indicated antibodies.