Anti-alpha kinase 1 antibodies are used in the immunodetection of the protein encoded by the ALPK1 gene. In humans, the canonical protein has a reported length of 1244 amino acid residues and a mass of 138.9 kDa. Its subcellular localization is in the cytoplasm. Alternative splicing is reported to yield 2 different isoforms for this protein. It is reported to be highly expressed in liver. A member of the Alpha-type protein kinase protein family, it is a type of serine/threonine-protein kinase that detects bacterial pathogen-associated molecular pattern metabolites (PAMPs) and initiates an innate immune response, a critical step for pathogen elimination and engagement of adaptive immunity. Other names for this target antigen include chromosome 4 kinase, lymphocyte alpha-kinase, lymphocyte alpha-protein kinase, and alpha-protein kinase 1.