Cell Signaling Technology
Phospho-p70 S6 Kinase (Thr389) (D5U1O) Rabbit
97596
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My research focus is on understanding the role of the immune system in regulating blood glucose and adipose tissue. For this experiment, mouse CD4+ T-cells were stimulated or stimulated with rapamycin. Rapamycin treatment inhibited S6K1 phosphorylation. P-S6K1 is a good readout for mTOR signaling.
Western Blot
CD4+ mouse T-cells
4% BSA in TBST overnight at 4°C
5% milk in TBST
5% milk in TBST for 2 hours at room temperature
N/A
ECL
Mouse CD4+ T-cells were non stimulated (left 4), stimulated (middle 4) or stimulated with rapamycin (right 4). Rapamycin treatment inhibited mTOR signaling by decreasing the phosphorylation of S6K1.
Nice clear band
Weak signal
This is a good antibody to interrogate mTOR signaling. However, the signal can be a little weak.