CST Antibody Against Phospho-p70 S6 Kinase Works Very Well

Cell Biology
Harvard Medical School
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Phospho-p70 S6 Kinase

Catalog Number:

9234

I work on selective forms of autophagy, especially focusing on ribophagy and ER-phagy. I have used large numbers of antibodies for proteins related to autophagy. This Phospho-p70 S6 Kinase antibody is great. There are no background bands.

Experimental Design and Results Summary

Applications

Western Blot

Sample

293T, HCT116

Primary Incubation

1:1000, overnight

Blocking Agent

5% non-fat milk

Secondary Incubation

1:20000 odyssey IR800

Tertiary Incubation

In TBS buffer.

Detection

Odyssey CLX

Results Summary

Works very well.

DOI or PMID #

10.1016/j.molcel.2019.03.034

Additional Notes

N/A

Image Gallery

Summary

The Good

No background, high specificity

The Bad

Nothing

The Bottom Line

I highly recommend this antibody.

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