Cell Signaling Technologies (CST)
Phospho-p70 S6 Kinase (Thr389) Antibody
9205
Info:Supplier Page View Product SpecsView Product
Browse Similar Products: Phospho-p70 S6 Kinase Western Blot Antibodies
Our lab studies the role of mTOR signaling in T cell such as mTORC1 signaling during T cell differentiation and function. With immunoblot analysis, we assessed pS6K1 (T389) activity as a direct readout of mTORC1 signaling in WT T cells upon T cell activation.
Western Blot
Murine T cells with stimulation
1:2000 in 4%BSA/TBST 4 deg overnight shaking
5% milk/TBST
1:5000 anti-Rabbit HRP (CST) in 5%milk/TBST
None
UVP Imager
We are readily able to detect mTORC1 activity by assessing pS6K1 (T389) in WT T cells upon stimulation through immunoblot analysis.
N/A
Use rapamycin to determine true band for first time use.
Consistent antibody. Can reuse many many times.
Great mTORC1 signaling readout antibody for western blotting.