Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199) Antibody

July 31, 2017

BlueskyReddit
Biophysics
National Institute Of Nutrition, Hyderabad
Ph.D Scholar

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Phospho-PI3 Kinase p85 (Tyr458)/p55 (Tyr199)

Catalog Number:

4228

I used this antibody for a signalling pathway study and to see whether there is some factor activating pi3k or not.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Caco2 cell lysate

Primary Incubation

1:1000 dilution, overnight at 4 degrees

Blocking Agent

5% Bovine serum albumin dissolved in 1%TBST

Secondary Incubation

1:5000 dilution in 1x TBST, 1hr, room temperature

Tertiary Incubation

None

Detection

ECL substrate used developed by G-BOX chemiluminiscent

Results Summary

I got results from this antibody but they are not good. It gives multiple bands and high background.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Bands will come

The Bad

Multiple bands and high background

The Bottom Line

This antibody has two molecular weights. It gives multiple bands and a high background.

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