Relatively Good Antibody for p-S6K

June 22, 2020

Oncology
Johns Hopkins School of Medicine
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Phospho-p70 S6 Kinase (Thr389) (108D2) Rabbit mAb #9234

Catalog Number:

9234

Clone Number:

108D2

We are interested in understanding how mTOR signaling regulates lymphocyte activation and differentiation.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse lymphocyte lysates

Primary Incubation

1:2500 in 4% BSA

Blocking Agent

5% milk

Secondary Incubation

1:5000 in 5% milk

Tertiary Incubation

None

Detection

ECL

Results Summary

Good signal most of the time.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Good signal

The Bad

None

The Bottom Line

Might need to titrate the antibody for the best signal to noise ratio.

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