Great Phospho-AMPK Alpha Antibody from Genetex

September 27, 2021

Department of Biomedical Imaging and Radiological Sciences
National Yang Ming Chiao Tung University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Genetex

Product Name:

AMPK alpha 1 (phospho Thr183) + AMPK alpha 2 (phospho Thr172) antibody

Catalog Number:

GTX52341

Clone Number:

N/A

Info: Supplier Page View Product Specs

Browse Similar Products: Anti-AMPK Antibodies

We used this antibody to observe the protein expression of phospho-AMPK alpha.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Huh7

Primary Incubation

1:1000 O/N at 4 degree

Blocking Agent

5% BSA in TBST

Secondary Incubation

1:10000 for 1 hr at RT

Tertiary Incubation

N/A

Detection

HRP substrate

Results Summary

phospho-AMPK alpha was increased after treating sorafenib.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Strong antibody signal, high specificity and clean background

The Bad

The antibody signal is too strong so we need to pay attention to the dilution ratio

The Bottom Line

The antibody has a great signal, highly recommended!

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