Anti-F4/80 antibodies are essential reagents in flow cytometry for detecting the target protein, adhesion G protein-coupled receptor E1. Encoded by the ADGRE1 gene in humans, this membrane receptor protein is involved in cell adhesion and interactions between immune cells. Human EMR1 is used as a marker for eosinophils, whereas F4/80 in mice is used as a marker for macrophages. Examine the supplier's provided validation data when comparing F4/80 antibodies for flow cytometry. Also look for application-specific recommendations, such as dilutions, protocols, sample types, and other helpful usage notes.