RAT ANTI MOUSE F4/80 MCA497GA from Bio-Rad (Formerly AbD Serotec)

Product Specs
ItemRAT ANTI MOUSE F4/80
CompanyBio-Rad
Price $323.00Supplier Page View Company Product Page
Catalog NumberMCA497GA
NCBI Gene AliasesEmr1, DD7A5-7, Ly71, Gpf480, F4/80, EGF-TM7, TM7LN3
CloneCl:A3-1
TargetF4/80
ApplicationsFlow Cytometry,Immunofluorescence,Immunohistology - Frozen,Western Blotting,Immunohistology - Paraffin,Immunoprecipitation,Radioimmunoassays,Immunohistology - Resin,Immuno-electron Microscopy
Quantity0.1 mg
IsotypeIgG2b
FormatPurified IgG - liquid
HostRat
ReactivityMouse
ClonalityMonoclonal Antibody
NCBI Full Gene Nameadhesion G protein-coupled receptor E1
ConcentrationIgG concentration 1.0 mg/ml

Questions and Answers

Can this antibody be used for detecting macrophages in tissue samples?

Yes, the rat anti-mouse F4/80 antibody (MCA497GA) has been utilized in various studies for the detection of macrophages in immunohistochemistry and immunofluorescence applications. For instance, it has been employed to identify F4/80+ macrophages in adipose tissue and in kidney specimens, demonstrating its effectiveness in visualizing macrophage populations in different tissue contexts.

Is there any evidence of this antibody being used in studies related to kidney injury?

Yes, the F4/80 antibody has been referenced in studies investigating kidney injury. For example, it was used in immunohistochemical staining to assess macrophage infiltration in models of acute kidney injury, highlighting its role in evaluating inflammatory responses in renal tissues.

What applications has this antibody been shown to work in according to the literature?

The rat anti-mouse F4/80 antibody has been shown to work in several applications, including Western blotting, flow cytometry, immunofluorescence, immunohistochemistry, and immunoprecipitation. Its versatility makes it suitable for various experimental setups aimed at studying macrophage biology.

Are there specific experimental conditions or models where this antibody has been effectively utilized?

The F4/80 antibody has been effectively utilized in various experimental models, including studies on obesity-related inflammation in adipose tissue and in models of bone turnover and regeneration. It has been used to analyze macrophage activation and polarization in these contexts, indicating its relevance in diverse biological research areas.

Bio-Rad

Bio-Rad Laboratories, Inc. Life Science Research Group 2000 Alfred Nobel Drive

Hercules, California 94547

United States

Phone: 800-424 6723
800-879 2289

Company Profile

Website: http://discover.bio-rad.com

(1) Systemic microbial antigen administration ameliorates experimental autoimmune encephalomyelitis via MHC-II downregulation in the CNS and secondary lymphoid organsJournal of Neuropathology & Experimental NeurologyApril 1, 2026Marina Boziki, Paschalis Theotokis, Evangelia Kesidou, Eleni Karafoulidou, Ilias Salamotas, Anastasia Chatziefstratiadou, Olga Touloumi, Konstantinos Papadakos, Dionyssios Sgouras, Ofira Einstein, et al.incubated with primary antibodies at 4°C overnight. Primary antibodies used were rat Anti-F4/80, BioRad MCA497GA, dilution 1:200; mouse Anti-MHCII, Abcam, ab55152, dilution 1:1000; and rat Anti-CD45R, BD Pharmingen,

(2) The CXCR2–XBP1–NF-κB Axis Drives Inflammatory Amplification and Tissue Damage in Acute Lung InjuryInflammationMarch 23, 2026Zhiyi Liu, Qiong Wu, Xing Liu, Tiantian Ke, Zhisu Yan, Lei Xiong, Fang Tangcollagen. Immunohistochemistry (IHC) and Immunofluorescence (IF) Sections were incubated with anti-F4/80 (Bio-Rad, MCA497GA; 1:200), anti-CD86 (Cell Signaling, 91882; 1:200), or anti-CD206 (Abcam, ab64693; 1:200). Nuclei were

(3) Expansion of bone marrow adipocytes in obese mice leads to PD-L1-driven bone marrow immunosuppression and osteoclastogenesisBone ResMarch 20, 2026Samantha N. Costa, Carolyn Chlebek, Lindsey Gray, Peter Caradonna, Sergey Ryzhov, Clifford J. Rosengoat serum (Abcam ab7481) in 0.1% PBS-Triton X100 for 30 minutes at RT. The F4/80 antibody (BioRad MCA497GA) was diluted 1:2 000 in blocking buffer, then slides were incubated with the antibody overnight at 4°C.

(4) The therapeutic effects of the VEGF decoy receptor fusion protein VEGF-Grab in chronic inflammatory diseasesEBioMedicineMarch 19, 2026Youngeun Lee, Hee-Young Lee, Hye Seong Lim, Jin A. Shin, Hyun Gul Yang, Saseong Lee, Grazia Mercadante, Valeria Cicatiello, Sandro De Falco, Hyungmin Kim, et al.macrophage infiltration, immunohistochemical staining was performed using an anti-F4/80 Ab (catalog MCA497GA; Bio-Rad, Hercules, CA). F4/80-positive cells were manually counted in three random fields per section. The

(5) A hierarchical theranostic nanoagent for multimodal imaging and targeted foam cell intervention in atherosclerosisNature CommunicationsMarch 11, 2026Jianwen Song, Xiaoying Kang, Shuxuan Yang, Xue Meng, Zihang Shi, Wen Li, Ji Qiimmunofluorescence, sections were rinsed, blocked, and incubated with anti-F4/80 primary antibody (1:500, Bio-Rad, MCA497GA), followed by washing and incubation with fluorescently labeled secondary antibody for 60 min at room

(6) Deficiency of exocyst complex component Exoc5 exacerbates the progression of kidney fibrosisExperimental & Molecular MedicineMarch 4, 2026Hui Jae Lim, Yong Kwon Han, Mi Ra Noh, You Ri Park, Se Young Jang, Joshua H. Lipschutz, Kwon Moo ParkNa+/K+ ATPase (cat. no. ab76020, Abcam), SGLT2 (cat. no. 24654-1-AP, Proteintech) and F4/80 (cat. no. MCA497GA, Bio-Rad Laboratories) antibodies. The quantification of F4/80+ cells was performed by counting the

(7) Pyrimidinergic calcium signaling links tubular metabolism to fibrosis in kidney diseaseNature CommunicationsFebruary 19, 2026Andreja Figurek, Nevena Jankovic, Sarah Kollar, Monika Kaminska, Imene Sakhi, Anna Rinaldi, Pietro E. Cippà, Bernard Robaye, Andrew M. Hallanti-Fibronectin (Sigma, F3648, 1:200); mouse anti-Col1a1 (DSHB, F1C3, 1:100), rat anti-F4/80 (Bio Rad, MCA497GA, 1:50), rabbit anti-CDA (Thermo Fisher, PA5-76650, 1:100), mouse anti-Aquaporin 1 (Abcam, ab9566, 1:250).

(8) A macrophage-induced subpopulation of mesenchymal cells expressing Fcer1g contributes to wound-induced fibrosisNature CommunicationsFebruary 12, 2026Xinyi Ma, Ergang Wang, Vijitha Puviindran, Ziyuan Su, Xiaoxi Liu, Eijiro Shimada, Chengsong Yan, Yining Liu, Zhenyu Li, Puvi Nadesan, et al.1xPBS overnight at 4 °C prior to addition of the following primary antibodies: Rat-F4/80 (Biorad, Cat#MCA497GA, clone A3-1, 1:500), Rabbit-Fcer1g (Invitrogen, Cat#PA5-115222, 1:200), Goat-Pdgfra (R&D, Cat#AF1062;

(9) The transition from monocyte to tissue-resident macrophage requires DHPSNatureJanuary 21, 2026Gustavo E. Carrizo, Pianpian Lin, Seung Hyun Lee, Kevin Shenderov, Camille Blériot, Minsun Cha, Lena Schimmelpfennig, Zhen Shen, Nikki van Teijlingen Bakker, Katarzyna M. Grzes, et al.antibodies were used: anti-PDGFRα (R&D Systems, AF1062, polyclonal, 1:200 dilution), anti-F4/80 (Bio-Rad, MCA497GA or MCA497B, clone Cl:A3-1, 1:300 or 1:50), anti-CD64 (Invitrogen, MA5-29706, clone 027, 1:300), anti-GFP

(10) Living sensor display implanted on skin for long-term biomarker monitoringNature CommunicationsJanuary 12, 2026Jun Sawayama, Makoto Takeo, Yuki Takayama, Miki Takase, Hoshimi Aoyagi, Aki Takimoto, Saki Mizutani, Maiko Onuki, Satoshi Ikeo, Shogo Nagata, et al.Anti-Mitochondria antibody (Merck (Sigma)・MAB1273, Human Mitochondria), Rat Anti-Mouse F4/80 antibody (Bio-Rad・MCA497GA, F4/80), Anti-CD86 antibody (Cell Signalling Technology・ #19589S, CD86) Anti-CD163 antibody (abcam・ab182422,

(11) Mouse digit AAV gene delivery into fibroblasts regulates regenerative outcomeScience AdvancesJanuary 9, 2026Vivian Jou, Scott D. Semelsberger, Jack Smerczynski, Jessica A. Lehoczkyanti-VIM (1:200, Novus, NB300-233), anti-RUNX2 (1:200; Abcam, ab192256), anti-F4/80 (1:100; Bio-Rad, MCA497GA), anti-SP7 (1:2000; Abcam, ab22552). Tissues were incubated with appropriate Cyanine3 (Cy3)- or Alexa

(12) TGFβ signaling mediates microglial resilience to spatiotemporally restricted myelin degenerationNature NeuroscienceJanuary 2, 2026Keying Zhu, Yun Liu, Jin-Hong Min, Vijay Joshua, Jianing Lin, Yue Li, Judith C. Kreutzmann, Yuxi Guo, Wenlong Xia, Elyas Mohammadi, et al.goat-anti-IBA1 (1:400, ab5076, Abcam), rat-anti-MHC-II (1:200, 14-5321-82, Thermo), rat-anti-F4/80 (1:200, MCA497GA, Bio-Rad), rabbit-anti-GPNMB (1:500, ab188222, Abcam), rat-anti-Galectin-3 (1:200, 14-5301-82, Thermo),

(13) Forced polarisation of microglia by IL-13 is modified by inflammatory and microenvironmental contextInflammation ResearchDecember 1, 2025Emmanuelle D. Aiyegbusi, James P. Reynolds, Ross O’Carroll, Ruth Colbert, Christopher Carew, Dearbhaile Dooleyab180779), Anti-Iba1 (FujiFilm Wako, 019–19741), Anti-Arginase 1 (Abcam, ab91279) and Anti-F4/80 (BioRad, MCA497GA) were added at a dilution of 1:400 using an antibody diluent consisting of 1% protein block and 0.05%

(14) The retinol-binding protein receptor STRA6 and melanin cooperatively sustain retinoid signaling and outer blood-retinal barrier integrityJournal of Biological ChemistryDecember 1, 2025Aicha Saadane, Johannes von Lintigovernight at 4 °C with rabbit anti-IBA1 (MA5-36257; Invitrogen; 1:200 dilutions in blocking buffer), F/480 (MCA497GA; Bio-Rad, Radnor, PA; 1:200 in blocking buffer), rabbit anti-GFAP (3670P; 1:400), and anti-vimentin

(15) Peptidylarginine deiminase 2 contributes to pathogenesis in trinitrobenzenesulfonic acid-induced colitis through macrophage extracellular trap-independent pathwaysScientific ReportsNovember 18, 2025Hiroyuki Yasuda, Michiko Saito, Shusaku Hayashi, Shinichi Kato1:500, Abcam, ab5103), goat anti-MPO (2 µg ml−1, R&D, AF3667), or rat anti-F4/80 (1:1000, Bio-Rad, MCA497GA), respectively. The samples were then incubated with donkey anti-rabbit IgG (Alexa Fluor 594, 1:800;

(16) Comparative analysis of LJ-4378 and tirzepatide in mouse models of obesity and weight regainArchives of Pharmacal ResearchNovember 7, 2025Heeseong Kim, Seung Woo Kim, Cheoljun Choi, SungUg Joo, Gibae Kim, Minjae Kim, Eunsuk Yang, Jangwon Lee, Sukjae Chung, Lak Shin Jeong, et al.5.10.00). Immunohistochemistry was performed on deparaffinized gWAT sections using an anti-F4/80 antibody (1:500, MCA497GA, Bio-rad). After overnight incubation with the primary antibody, sections were incubated for 1 h at

(17) Perivascular inflammation in the progression of aortic aneurysms in Marfan syndromeJCI InsightOctober 8, 2025Hiroyuki Sowa, Hiroki Yagi, Kazutaka Ueda, Masaki Hashimoto, Kohei Karasaki, Qing Liu, Atsumasa Kurozumi, Yusuke Adachi, Tomonobu Yanase, Shun Okamura, et al.(Cell Signaling Technology, #9101), and rat monoclonal anti-F4/80 antibody (Bio-Rad Laboratories, Inc., MCA497GA). Vectastain ABC kit (Vector Laboratories, Inc.) and DAB Peroxidase Substrate Kit (Vector Laboratories,

(18) Immunoregulatory Roles of Tumor‐Originated Pericytes Identified by Single‐Cell Analysis in GlioblastomaAdvanced ScienceSeptember 26, 2025Cuiying Chu, Fangzhen Li, Zhiwen Zhang, Qiuhong Zhu, Hong Yan, Mengdan Cheng, Huipeng Wang, Lei Cheng, Zhe Zhang, Xingjiang Yu, et al.(Cell Signaling Technology, 9661S), Iba1 (Abcam, ab5076), CD163 (SANTA CRUZ BIOTECHNOLOGY, sc‐20066), F4/80 (Bio‐rad, MCA497GA), Arg1 (SANTA CRUZ BIOTECHNOLOGY, sc‐166920) were used for the staining of GBM tumor sections as indicated.

(19) Imeglimin attenuates liver fibrosis by inhibiting vesicular ATP release from hepatic stellate cellsFEBS LettersSeptember 15, 2025Seiji Nomura, Lixiang Wang, Nao Hasuzawa, Ayako Nagayama, Sawako Moriyama, Kenji Ashida, Yoshinori Moriyama, Masatoshi Nomura, Ken Yamamotoα‐smooth muscle actin (α‐SMA, 1 500 dilution, A5228; Sigma‐Aldrich, St. Louis, MO, USA), F4/80 (1 : 500, MCA497GA; Bio‐Rad AbD Serotec, Kidlington, Oxford, UK), and a polyclonal antibody against desmin (1:200, 16520‐1;

(20) Locally Reprogramming Tumor-Associated Macrophages with Cytokine-Loaded Injectable Cryogels for Breast CancerAnnals of Biomedical EngineeringAugust 29, 2025Sydney R. Henriques, Evan B. Glass, Kristen L. Hoek, Ori Z. Chalom, Abigail E. Manning, Sohini Roy, Diana K. Graves, Sarah M. Goldstein, Benjamin C. Hacker, Renjie Jin, et al.antibody labeling. The blocking buffer was removed, and 100 µL of 1:100 dilution of rat anti-mouse F4/80 (Bio-Rad; MCA497GA, Clone Cl:A3-1) was added and incubated at 4 °C overnight. The cells were washed again before permeabilizing

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RAT ANTI MOUSE F4/80

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RAT ANTI MOUSE F4/80

RAT ANTI MOUSE F4/80

RAT ANTI MOUSE F4/80

RAT ANTI MOUSE F4/80 from Bio-Rad (Formerly AbD Serotec)

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RAT ANTI MOUSE F4/80 from Bio-Rad (Formerly AbD Serotec)

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