This BioLegend Antibody Is Great for Identifying F4/80+ Cells

April 30, 2020

Pathology
Stanford University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 421™ anti-mouse F4/80 Antibody

Catalog Number:

123137

Clone Number:

BM8

We wanted to study macrophages in the disease vs healthy state, so we used this antibody to identify our macrophage population.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of tumor cells

Primary Incubation

1:200

Blocking Agent

FcBlock

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSRII flow cytometer

Results Summary

The antibody was very useful for pulling out our macrophage population.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

The antibody is great for identifying F4/80+ cells

The Bad

None to mention

The Bottom Line

The antibody can pull out your macrophage population clearly and distinctly.

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