This BioLegend Antibody Stains F4/80+ Cells Very Well

October 25, 2019

Pathology
Stanford University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

APC/Cyanine7 anti-mouse F4/80 Antibody

Catalog Number:

123118

Clone Number:

BM8

We wanted to study macrophages in the diseased vs healthy state, so we used this antibody to identify F4/80+ cells in mice.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of mouse spleen cells

Primary Incubation

1:200

Blocking Agent

FcBlock

Secondary Incubation

None

Tertiary Incubation

None

Detection

LSRII flow cytometer

Results Summary

The antibody stains F4/80+ macrophages very clearly.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

The antibody stains F4/80+ cells well

The Bad

None to mention

The Bottom Line

The antibody is great for identifying macrophages in mice.

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