Good Antibody as Far as F4/80 Staining Goes

Department of Pediatrics
University of California, San Diego
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

APC anti-mouse F4/80 Antibody

Catalog Number:

123116

Used for flow cytometry. Flow detection of F4/80 isn't always the best or clearest staining in my hands, it kind of depends on the cells / tissue analyzed, but this Ab is very good as far as F4/80 staining goes.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine lung or peritoneal exudate cells

Primary Incubation

1:200 4C 20 min in FACS buffer

Blocking Agent

FcBlock

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

APC flow cytometry

Results Summary

Looks good, clear populations can be detected. Sample shown is from peritoneal exudate cells, sometimes when I look in lung the populations are less distinct. But this works as well as any other F4/80 Ab that I've tried.

Additional Notes

None

Related Categories

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Summary

The Good

Easy use for flow

The Bad

Sometimes populations aren't distinct

The Bottom Line

Works as expected

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