Biolegend
Anti-mouse F4/80 APC
123116
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Browse Similar Products: F4/80 Flow Cytometry Antibodies
This antibody was used to stain macrophages in mouse peripheral blood. It was chosen because it is a commonly used clone, a bright fluorochrome, and Biolegend had the best price.
Flow Cytometry
ACK-lysed peripheral mouse blood
1uL per test in 100uL total staining volume for 20 minutes at room temperature
1:50 dilution of anti-mouse FcBlock and 2% FBS.
n/a
Macrophage staining was low, but as expected, in peripheral blood.
None
Works well. Low cost
Will use again.